[Album] hana*hana – GOLDEN BEST SINGLE COLLECTION [FLAC + MP3] 花*花 – GOLDEN BEST SINGLE COLLECTION Released: 2011 Genre: Pop Bitrate: FLAC & MP3 320 KBPS RAR / 450 MB; RAR / 165 MB Tracklist: 1 あ～よかった -setagaya mix- / 花*花 2 ずっと一緒に / 花*花 3 ひまわりの花 / 花*花 4 赤い自転車 / 花*花 5 さよなら 大好きな人 / 花*花 6 愛する人よ / 花*花 7 ハナムケノハナタバ / 花*花 8 秘密基地 / 花*花 9 やっぱり! / 花*花 10 あなたへ.

/ 花*花 11 愛を少し語ろう / 花*花 12 ばんそうこう1枚 / 花*花 13 涙のチカラ / 花*花 14 晴れた夜 雨の午後 / 花*花 15 LONELY GIRL / 花*花 16 ポロポロ / 花*花 17 カプチーノ / 花*花 18 植物園北門前 (funk version) / 花*花.

Abstract Hyperplastic candidiasis is characterized by thickening of the mucosal epithelia with Candida albicans infection with occasional progression to squamous cell carcinoma (SCC). Albicans is a critical factor in tumor development; however, the oncogenic mechanism is unclear. We have previously produced an animal model for hyperplastic candidiasis in the rat forestomach. In the present study, we investigate whether impaired DNA methylation and associated protein expression of tumor suppressor and DNA repair genes are involved in the SCC carcinogenesis process using this hyperplastic candidiasis model. Promoter methylation and protein expression were analyzed by methylation specific PCR and immunohistochemical staining, respectively, of 5 areas in the forestomachs of alloxan-induced diabetic rats with hyperplastic candidiasis: normal squamous epithelia, squamous hyperplasia, squamous hyperplasia adjacent to SCC, squamous hyperplasia transitioning to SCC, and SCC. We observed nuclear p16 overexpression despite increases in p16 gene promoter methylation during the carcinogenic process.

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TIMP3 and RAR-β2 promoter methylation progressed until the precancerous stage but disappeared upon malignant transformation. In comparison, TIMP3 protein expression was suppressed during carcinogenesis and RAR-β2 expression was attenuated in the cytoplasm but enhanced in nuclei. ERCC1 and BRCA1 promoters were not methylated at any stage; however, their protein expression disappeared beginning at hyperplasia and nuclear protein re-expression in SCC was observed only for ERCC1. These results suggest that aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 expression might occur that is inconsistent with the respective gene promoter methylation status, and that this overexpression might serve to promote the inflammatory carcinogenesis caused by C. Albicans infection. Citation: Terayama Y, Matsuura T, Ozaki K (2016) Lack of Correlation between Aberrant p16, RAR-β2, TIMP3, ERCC1, and BRCA1 Protein Expression and Promoter Methylation in Squamous Cell Carcinoma Accompanying Candida albicans-Induced Inflammation.